2×Taq PCR MasterMix Ⅱ is a newly optimized and upgraded ready-to-use 2×PCR premix with all the essential parts in PCR reaction except DNA template and primers.
■ High amplification efficiency: DNA fragments of different sizes (lower than 5 kb) and sources can be amplified efficiently. ■ High sensitivity: As low as 10 pg of target fragments can be amplified from genomic templates. ■ High stress resistance: For templates with high impurity content such as rough-extracted template/bacterial culture, the target fragment can be easily amplified. The polymerase activity will not be affected by repeated freezing and thawing. ■ Convenient for applications: The reaction system was prepared easily and quickly. The amplified fragment contains the 3' end dA-overhang, which is convenient for TA cloning.
Specification
Type: Taq DNA polymerase Sample: Purified/rough-extracted template/bacterial culture Template: >10 pg Fragment size: <5 kb Applications: PCR amplification of DNA fragments, DNA labeling, primer extension, sequence determination, large-scale gene detection, semi-quantitative PCR experiments, detection of trace DNA, etc.